公鸡弱精症相关候选基因的表达分析

doi:10.11843/j.issn.0366-6964.2015.06.003

Abstract

Abstract:

To explore the pathogenesis of the rooster asthenospermia，the expression profiles of 6 candidate genes (COX7B，PTGDS，hPGDS，SPAG6，WNT2 and CCNF) selected from the previous digital gene expression profiling (DGE) and bioinformatics analysis，were analyzed.A total of 300 Beijing-You roosters of 42 weeks were detected for 4 weeks for semen quality including semen volume，sperm mobility，etc.Fifteen asthenospermia and 15 normal roosters were selected accordingly.Real-time quantitative PCR were performed to determine the expression of the 6 genes in the testicular of those roosters.The results indicated that COX7B and PTGDS were significantly high-expressed in the asthenospermia group (P<0.01)，while the WNT2，hPGDS and CCNF were significantly low-expressed in the asthenospermia group (P<0.05).However，the expression of SPAG6 gene was not significantly different in the 2 groups.These results were in accordance with the previous DGE analysis.The expression patterns of the 6 genes identified from DGE for asthenospermia in chickens were validated in the present study.They can be important candidate genes for further functional study and revealing the mechanism of chicken asthenospermia.

CLC Number:

S831
S813.3

FU Li，SUN Yan-yan，XUE Fu-guang，LIU Ran-ran，BAI Hao，CHANG Guo-bin，CHEN Guo-hong，CHEN Ji-lan. The Expression of the Six Candidate Genes for Asthenospermia of the Rooster[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 889-895.

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The Expression of the Six Candidate Genes for Asthenospermia of the Rooster

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